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<nowiki>Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent. DTT's formula is C4H10O2S2 and the molecular structure of its reduced form is shown at the right; its oxidized form is a disulfide-bonded 6-membered ring (shown below). Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol. A common use of DTT is as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors. Typically DTT is mixed with a DNA solution and allowed to react, and then is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form). The DTT removal procedure is often called "desalting.". DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. However, even DTT cannot reduce buried (solvent-inaccessible) disulfide bonds, so reduction of disulfide bonds is sometimes carried out under denaturing conditions (e.g., at high temperatures, or in the presence of a strong denaturant such as 6 M guanidinium hydrochloride, 8 M urea, or 1% sodium dodecylsulfate). Conversely, the solvent exposure of different disulfide bonds can be assayed by their rate of reduction in the presence of DTT. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione. In very rare cases, a DTT adduct may be formed, i.e., the two sulfur atoms of DTT may form disulfide bonds to different sulfur atoms; in such cases, DTT cannot cyclize since it has no remaining free thiols. Due to air oxidation, DTT is a relatively unstable compound whose useful life can be extended by refrigeration and handling in an inert atmosphere. Since protonated sulfurs have lowered nucleophilicities, DTT becomes less potent as the pH lowers. Tris(2-carboxyethyl)phosphine HCl (TCEP hydrochloride) is an alternative which is more stable and works even at low pH.</nowiki><br>
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Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent. DTT's formula is C4H10O2S2 and the molecular structure of its reduced form is shown at the right; its oxidized form is a disulfide-bonded 6-membered ring (shown below). Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol. A common use of DTT is as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors. Typically DTT is mixed with a DNA solution and allowed to react, and then is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form). The DTT removal procedure is often called "desalting.". DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. However, even DTT cannot reduce buried (solvent-inaccessible) disulfide bonds, so reduction of disulfide bonds is sometimes carried out under denaturing conditions (e.g., at high temperatures, or in the presence of a strong denaturant such as 6 M guanidinium hydrochloride, 8 M urea, or 1% sodium dodecylsulfate). Conversely, the solvent exposure of different disulfide bonds can be assayed by their rate of reduction in the presence of DTT. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione. In very rare cases, a DTT adduct may be formed, i.e., the two sulfur atoms of DTT may form disulfide bonds to different sulfur atoms; in such cases, DTT cannot cyclize since it has no remaining free thiols. Due to air oxidation, DTT is a relatively unstable compound whose useful life can be extended by refrigeration and handling in an inert atmosphere. Since protonated sulfurs have lowered nucleophilicities, DTT becomes less potent as the pH lowers. Tris(2-carboxyethyl)phosphine HCl (TCEP hydrochloride) is an alternative which is more stable and works even at low pH.
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<nowiki>'''とある魔術の禁書目録のヒロインの一人であり、とある科学の超電磁砲の主人公。 『超電磁砲（レールガン）』の異名を持つ御坂美琴のスレです。 彼女の恋が成就するように祈りつつ、ﾏﾀｰﾘ語りましょう。 CV：佐藤利奈'''</nowiki> <br>
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'''とある魔術の禁書目録のヒロインの一人であり、とある科学の超電磁砲の主人公。『超電磁砲（レールガン）』の異名を持つ御坂美琴のスレです。 彼女の恋が成就するように祈りつつ、ﾏﾀｰﾘ語りましょう。 CV：佐藤利奈'''
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<nowiki>''蓝绿空间比例不低于70%，新建绿色建筑比例将达到100%，新建民用建筑装配式比例不小于30%，住宅、公建新能源汽车充电车位比例分别不小于10%、15%……以绿色为底色、瞄准全国最大绿建产业示范区的济南新旧动能转换先行区。4月29日，济南新旧动能转换先行区管委会在官网发布《济南新旧动能转换先行区绿色生态建设指标体系》（以下简称《绿色生态建设指标体系》）、《济南新旧动能转换先行区建设项目绿色生态建设服务规程》（试行），将于5月1日起试行。''</nowiki><br>
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''蓝绿空间比例不低于70%，新建绿色建筑比例将达到100%，新建民用建筑装配式比例不小于30%，住宅、公建新能源汽车充电车位比例分别不小于10%、15%……以绿色为底色、瞄准全国最大绿建产业示范区的济南新旧动能转换先行区。4月29日，济南新旧动能转换先行区管委会在官网发布《济南新旧动能转换先行区绿色生态建设指标体系》（以下简称《绿色生态建设指标体系》）、《济南新旧动能转换先行区建设项目绿色生态建设服务规程》（试行），将于5月1日起试行。''
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<nowiki>'''''感谢您的咨询。南部山区教师编招聘公告发布时间大约为七月中旬，计划为100人（具体需要审批），招聘范围为中小学教师，岗位要求本科及以上学历，考试科目为教育综合知识和学科专业知识。公告将第一时间在南部山区管委会官方网站发布，敬请关注。'''''</nowiki><br>
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'''''感谢您的咨询。南部山区教师编招聘公告发布时间大约为七月中旬，计划为100人（具体需要审批），招聘范围为中小学教师，岗位要求本科及以上学历，考试科目为教育综合知识和学科专业知识。公告将第一时间在南部山区管委会官方网站发布，敬请关注。'''''
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<nowiki><font color="#0000FF">开展苏州市青少年科技节，探索启动 STEM全市教育项目。深化教体融合，完善初中毕业生体育考试方案。举办了苏州市“青少年校园足球特色学校”校长论坛，承办了江苏省足球特色幼儿园现场观摩活动。新增全国青少年校园足球特色学校46所，省青少年校园足球试点县（区）2个，目前全市共有全国特色学校198所，省特色幼儿园9所，数量位居全省第一。在江苏省第十九届省运会上，我市青少年部代表队取得了金牌总数第一、奖牌总数第一、团体总分第一的好成绩。在2018年全省第六次中小学生艺术展演中，苏州获得十个类别中4个特等奖第一名，名列全省第一。新增6所江苏省艺术教育特色学校。苏州市中小学社会实践统一管理平台正式启用，实现了学生社会实践活动记录的信息化、规范化管理。与文明办联合开展“家在苏州 E路成长”未成年人社会实践体验成果展示，在112个体验站基础上2018年又新增16个体验站，结成48对站校联盟，推出32个配套课程。推进品格教育，2018年新增省品格工程项目4个，市项目16个，1个项目获评2018年全国中小学德育工作典型经验。成立第二批18个班主任名师工作室，开展全市初任班主任系统培训，省班主任基本功竞赛再获佳绩。优化心育服务体系和保障体系，心理外援、心理教研活动、心理危机干预等机制运行良好,全面推进学校心理教育工作。完成苏州教育志愿者活动管理平台改版升级工作，学生志愿服务项目丰富，志愿服务时长不断提升。开展“百团大赞”学生社团活动，学校社团类型、覆盖度、活动频率都有较大提升。</font></nowiki><br>
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<font color="#0000FF">开展苏州市青少年科技节，探索启动 STEM全市教育项目。深化教体融合，完善初中毕业生体育考试方案。举办了苏州市“青少年校园足球特色学校”校长论坛，承办了江苏省足球特色幼儿园现场观摩活动。新增全国青少年校园足球特色学校46所，省青少年校园足球试点县（区）2个，目前全市共有全国特色学校198所，省特色幼儿园9所，数量位居全省第一。在江苏省第十九届省运会上，我市青少年部代表队取得了金牌总数第一、奖牌总数第一、团体总分第一的好成绩。在2018年全省第六次中小学生艺术展演中，苏州获得十个类别中4个特等奖第一名，名列全省第一。新增6所江苏省艺术教育特色学校。苏州市中小学社会实践统一管理平台正式启用，实现了学生社会实践活动记录的信息化、规范化管理。与文明办联合开展“家在苏州 E路成长”未成年人社会实践体验成果展示，在112个体验站基础上2018年又新增16个体验站，结成48对站校联盟，推出32个配套课程。推进品格教育，2018年新增省品格工程项目4个，市项目16个，1个项目获评2018年全国中小学德育工作典型经验。成立第二批18个班主任名师工作室，开展全市初任班主任系统培训，省班主任基本功竞赛再获佳绩。优化心育服务体系和保障体系，心理外援、心理教研活动、心理危机干预等机制运行良好,全面推进学校心理教育工作。完成苏州教育志愿者活动管理平台改版升级工作，学生志愿服务项目丰富，志愿服务时长不断提升。开展“百团大赞”学生社团活动，学校社团类型、覆盖度、活动频率都有较大提升。</font>